Mutants of Arabidopsis thaliana Hypersensitive to DNA-Damaging Treatments
نویسندگان
چکیده
منابع مشابه
کلن کردن ژنRGL2 از DNA ژنومی آرابیدوپسیس (Arabidopsis thaliana)
یکی از ژنهایی که در سنتز جیبرلین در گیاه شناخته شده و موقعیت آنها در ژنوم آرابیدپسیس مشخص شده است، RGL2 می‌باشد که در این بررسی کلن گردید. با استخراج DNA از آرابیدوپسیس، اکوتیپ کلمبیا و استفاده از آغازگرهای اختصاصی که بر اساس توالی ژن مورد نظر ساخته شده بودند، این ژن تکثیر شد. پلاسمید PET-32a (+)و ژن تکثیر شده با استفاده از دو آنزیم برشی خاص به نامهای Nco I و Sal I برش داده شدند. پس از اتصال ژ...
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ژورنال
عنوان ژورنال: Genetics
سال: 1997
ISSN: 1943-2631
DOI: 10.1093/genetics/146.1.401